Here are suggested steps for addressing this analysis: 1. What do you think about using numbers of cells for defining yields and rates, as exemplified above? Provide specific justifications whatever your position is. 2. Whatever your assessment is in the above question, suppose that you want to explore another avenue for reporting the parameters other than based on cell numbers, how would you suggest to report the data? 3. Suppose that you are asked to re-examine the published literature relevant to the cell culture program you are involved with at your Company, and to translate all yields and rates based on number of cells to the improved analysis you have proposed in Question #2. What are the steps (including type of experiments and calculations) you would perform in order to derive the parameters in the new format? 4. Following Question #3, provide a specific quantitative determination of μ, YX/O2 and qS, using information presented in this Module or previous Modules, or based on other references (please cite, in such a case).
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