SUPPOSE THAT YOU WANTED TO USE BACTERIA TO EXPRESS THE PRODUCT OF A EUKARYOTIC GENE. GIVEN THE DIFFERENCES BETWEEN BACTERIAL AND EUKARYOTIC MRNA PRODUCTION COVERED IN TOPIC 9 WHY WOULD YOU USE A CDNA CLONE INSTEAD OF A GENOMIC DNA CLONE?

SUPPOSE THAT YOU WANTED TO USE BACTERIA TO EXPRESS THE PRODUCT OF A EUKARYOTIC GENE. GIVEN THE DIFFERENCES BETWEEN BACTERIAL AND EUKARYOTIC MRNA PRODUCTION COVERED IN TOPIC 9 WHY WOULD YOU USE A CDNA CLONE INSTEAD OF A GENOMIC DNA CLONE?

 

 

WHAT WOULD A BACTERIAL EXPRESSION VECTOR NEED TO HAVE BESIDES THE SITE TO INSERT THE CDNA?

Suppose that you wanted to use bacteria to express the product of a eukaryotic gene. Given the differences between bacterial and eukaryotic mRNA production covered in Topic 9, why would you use a cDNA clone instead of a genomic DNA clone? What would a bacterial expression vector need to have besides the site to insert the cDNA?

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