- Compare the amount of time to acquire spinning disk confocal images with the time to take Airyscan confocal images? Consider the exposure times and image size and quality.
- Write 4 synopses and give reference citations for articles dealing with confocal and/or super-resolution microscopy.
- Compare the widths of the labeled striations in expansion microscopy, Airyscan super-resolution microscopy, and spinning disk confocal microscopy. Which provided the best resolution? What is your estimate of the increase in magnification from expansion microscopy based on the distance between C zones?
- How do epifluorescence and Airyscan confocal images compare in depth of field? What is the depth of field in expansion microscopy?
- Describe the procedures for expansion microscopy and Airy scan confocal microscopy.
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